Three people remain in a serious condition in the John Hunter Hospital after their car collided with a train at Wallarobba.

Emergency services were called a rural rail crossing about 15 kilometres south of Dungog just after 4.30pm on Tuesday to reports a Toyota Tarago had collided with a passenger train.

A 59-year-old woman who was driving the car and a 65-year-old female passenger suffered serious injuries, while a 65-year-old man was thrown from the vehicle and also suffered serious injuries.

They were treated by paramedics before being flown to hospital.

The train driver and his nine passengers escaped injury.

Police have set up a crime scene as investigations into the crash continue.

The crumpled wreck of the Tarago at Wallarobba. Picture 9News